CRISPR-modified Culex quinquefasciatus
Last year team CGRG genetically modified Hawai‘i sourced Culex quinquefasciatus (southern house mosquito) to test stable incorporation of components of our reversible gene drive system (note that we inserted two genes, not the full drive system). This work was Jared Nishimoto's MSc. thesis, which he defended in July 2019, and we also developed it into an undergraduate teaching lab module. We are currently completing construction of the full gene drive system, and plan to use CRISPR to insert it alongside an eye-color phenotype marker. We began using CRISPR in the lab a few weeks ago, and here are some early results: white-eyed Culex pupae. One photo is a wildtype shown for comparison - it has black eyespots. These photos are from G0s (injected eggs) so the next step is to rear them to see if we have a stable germline modification (our previous modification was germline stable).
All of this work is being done strictly in laboratory colonies.